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β-actin Rabbit Polyclonal Antibody

2022-03-29

品牌

貨號

名稱

規(guī)格

單價

說明書

EXPANDBIO

EP0051-100μL

β-actin Rabbit Polyclonal Antibody

100μL

980

EP0051.pdf

EXPANDBIO

EP0051-20μL

β-actin Rabbit Polyclonal Antibody

20μL

480



Applications

Species Cross-Reactivity

Molecular Weight

Isotype

WB, IHC, IF

H, R, M, Mk, Ch, Hm, Sh,Xenopus

43KD

IgG

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store at -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:3,000 IHC: 1:200 IF: 1:200

Optimal dilutions should be determined by the end user.

Specificity: Antibody can detects endogenous β-actin protein.

Background: β-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.

 

0051 1.png




0051 2.png 

Western blot analysis of HepG2 (1) , Rat liver (2) ,Mouse kidney (3) , Rabbit testic(4), Sheep lung(5), 293T(6) with β-Actin rabbit polyclonal antibody diluted at 1:5000.

0051 3.png 

IHC staining of Human ovary tissue with β-Actin PAB diluted at 1:200.

 

IF analysis of Hela with EP0051 diluted at 1:500.


 


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