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GAPDH Mouse Monoclonal Antibody(2B8)

2022-03-24

品牌

貨號(hào)

名稱

規(guī)格

單價(jià)

說明書

EXPANDBIO

EP0042-100μL

GAPDH Mouse Monoclonal Antibody(2B8)

100μL

980

EP0042.pdf

EXPANDBIO

EP0042-20μL

GAPDH Mouse Monoclonal Antibody(2B8)

20μL

480


 

Applications

Species Cross-Reactivity

Molecular Weight

Isotype

WB, IHC

H, R, M, Mk, Dg, Ch, Hm, Rb, Pg, Sh

Insect, Yeast

37KD

IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store at -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:5,000-20000  IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: Antibody can detects endogenous GAPDH protein.

Background: Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.


0042 1.png


 


Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb(2B8) diluted at 1:10,000. 





 

0042 2.png

 

Western blot analysis of Chicken Muscle (1), Hamster Lung (2), Pig Heart(3), 

with GAPDH mouse mAb(2B8) diluted at 1:10,000. 



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